DNA application including cloning, purification, PCR, gel electrophoresis.
Protein application and antibody application including ELISA a western blotting.
Tissue culture applications including preparing and maintaining viral stocks, plaque isolation, neutralization assays, primary cell development, cellular stocks, and transfections.
The applicant will be expected to perform nucleic acid extractions and real-time PCR and should ideally have experience in PCR of non-human viral target sequences.
40% laboratory management, which includes:
Ordering or shipping supplies
Organizing laboratory databases
Organizing and maintaining samples and reagents in freezers and refrigerators.
Assisting in the job responsibilities of other employees, assisting the hiring of personnel including training, assigning work and documenting the activities of other lab personnel
Performing equipment and computer maintenance
Assisting in maintaining regulatory compliance including overseeing inspections and assisting in protocol updates and maintenance.
All required qualifications must be documented on application materials
BS in microbiology, biochemistry, molecular biology, or a related field plus one year of research experience or an equivalent combination of education and relevant research experience totaling at least five years.
Required Experimental Skills:
Demonstrated experience using guinea pigs as animal models for vaccine therapy including sample collection, dissection, injection, colony maintenance, and hearing experiments.
Demonstrated experience with DNA applications including cloning purification, PCR, gel electrophoresis; protein applications and antibody applications including ELISA and western blotting; and tissue culture applications including preparing and maintaining stocks, plaque isolations, neutralization assays, primary cell development, cellular stocks, transfections.
Other Research Skills:
Demonstrated experience with laboratory management including purchasing, supervising undergraduate students, operating and maintaining standard laboratory equipment, shipping and receiving biological samples at national and international levels, and coordinating with regulatory agencies including FDA, USDA, IACUC, IRB, IBC.
Must be able to lift 50 lbs. in execution of daily duties.
We are looking for a person with experience in cytomegalovirus and using guinea pigs as an animal model that will perform both experimental and organizational duties in the laboratory. The successful candidate will be able to work independently, multi-task, and will have strong problem solving and organizational skills. In addition, the candidate must be able to effectively communicate in English and a have a flexible approach to job assignments. Experience in ordering supplies/equipment though the Universities system. Classes and/or training in subjects such as immunology, genetic microbiology, biochemistry, bioinformatics, and virology.
Internal Number: 340817
About University of Minnesota, Twin Cities
The University of Minnesota, founded in the belief that all people are enriched by understanding, is dedicated to the advancement of learning and the search for truth; to the sharing of this knowledge through education for a diverse community; and to the application of this knowledge to benefit the people of the state, the nation, and the world.