Research - Laboratory/Non-Laboratory, Staff/Administrative
75% Conduct experiments with human T cells in support of clinical applications. A broad range of activities will be expected, including: cell isolation (from umbilical cord blood, adult peripheral blood or mouse bone marrow cells); cell culture; flow cytometric characterization and purification (sorting); mouse injection, tissue harvesting, sectioning and staining; biochemical assays on DNA, RNA/protein.
15% Collect and integrate data from multiple sources. Review literature for relevant sources of scientific information and methods. Prepare summary reports of experimental results in support of manuscript preparation. Prepare graphics for presentation. Present summarized data to supervisor.
10% Documentation of experiments in data books (lab notebooks).
Required Qualifications (must be mentioned on application/resume): * Must be willing and able to handle human blood and lentiviral transduction of human cells.
1. BA/BS with at least 1 year of related experience or a combination of related education and work experience to equal five years; 2. Demonstrated experience successfully and independently performing multi-step experiments in a research lab per procedure in protocol or from verbal instructions of supervisor. 3. Demonstrated experience reporting effectively on the progress and direction of individual projects using written and verbal English, to include proper grammar usage and accurate spelling. 4. Demonstrated experience maintaining documentation and reports for data and procedures obtained from separate projects in an organized and logical manner. 5. Demonstrated experience using 3-laser flow cytometers and cell sorters (e.g. LSRII and FACSAria, respectively).
Preferred Qualifications: 1. Knowledge of immunology and murine models of disease. 2. Demonstrated experience assaying lymphocyte metabolism using the 'Seahorse' bioanalyzer from Agilent. 3. Previous experience working with in-vivo tumor or graft-vs-host disease models. 4. Ability to actively contribute to laboratory meetings and assist other laboratory members when needed.
Internal Number: 340624
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