Research - Laboratory/Non-Laboratory, Staff/Administrative
This is a temporary, full-time, grant supported position working 40 hours per week and expected to last up to 12 months with the possibility of extension based on satisfactory performance, availability of work, and departmental funding.
30% - Assist in gene and protein validation using quantitative RNA techniques such as quantitative (real time) reverse transcription polymerase chain reaction experiments (Q-PCR or RT-PCR) as well as assessing protein concentration using gel electrophoresis and western blotting techniques. Direct experience with these techniques preferred.
20% - Assist in flow cytometric experiments (FACS) analysis of myogenic precursor cell populations using a variety of markers. Direct experience with this technique preferred.
20% - Assist with physiological testing of muscle and eyes. Prepare solutions for drug administration. Assist with animal studies.
10% - Manage laboratory inventory. Work with one student employee on increasing skills using the physiology equipment.
15% - Perform morphometric and 3-dimensional reconstructions of muscle tissue produced by the experiments on muscle treatments, as well as keep the programs updated and calibrated. This involves tissue processing using a cryostat and immunohistochemical staining procedures. Maintain electronic systems for keeping laboratory inventory accurate.
5% Other duties as assigned.
The University of Minnesota offers a comprehensive benefits package including:
Competitive wages, paid holidays, vacation and sick leave
Low cost medical, dental, and pharmacy plans
Health care and dependent daycare flexible spending accounts
Excellent retirement plans with employer match
Disability and employer paid life insurance
Wellbeing program with reduced insurance premiums
Tuition reimbursement opportunities covering 75%-100% of eligible tuition
All required qualifications must be documented on application materials.
Bachelor’s degree and at least the equivalent of one (1) full-time year's work experience or an equivalent combination of education and relevant work experience to equal five (5) years.
Experience with molecular biology techniques such as genotyping, PCR, protein analysis using western blots.
Basic experience organizing and analyzing data.
Past experience assisting in maintaining detailed laboratory records.
Experience with histological processing.
Experience with morphometric analysis.
Experience with physiological testing of eyes.
Ability to carry out research protocols from written or oral instructions from lab director.
Ability to carefully follow directions and laboratory protocols and be detail oriented.
Ability to work well with others in the laboratory.
Some experience with flow cytometry.
Experience working individually and on group projects.
Review and summarize literature.
Communicate clearly and respectfully.
Demonstrated ability to follow directions carefully and work well in a collaborative environment.
Completion of RAR training for handling mice in SPF conditions.
Experience with microscopy, including confocal imaging and using a dissecting microscope.
Experience with animal surgery and tissue harvesting.
Experience in survival surgeries in mice, especially microsurgical techniques.
Knowledge and experience in anesthesia and analgesia.
Genotyping transgenic mice is highly desirable.
Conventional and confocal fluorescence microscopy, flow cytometry, and routinely used techniques in molecular biology.
Internal Number: 340573
About University of Minnesota, Twin Cities
The University of Minnesota, founded in the belief that all people are enriched by understanding, is dedicated to the advancement of learning and the search for truth; to the sharing of this knowledge through education for a diverse community; and to the application of this knowledge to benefit the people of the state, the nation, and the world.