Research - Laboratory/Non-Laboratory, Staff/Administrative
JOB SUMMARY: Plan and conduct research experiments as well as analyze results focusing on the construction, identification and characterization of genetically modified human cell lines. This includes, but is not limited to, the use of molecular biology techniques including designing DNA primers to be used in PCR for the construction of Cas/CRISPR gene targeting vectors, propagation and cloning of vectors in E. coli, as well as the extensive use of tissue culture techniques for human somatic cell lines. Additional techniques include DNA sequencing, Southern blot analysis, Western blot analysis and a bevy of molecular assays to assess the allelic status of human cells. Included in this category of conducting experiments is the use of computer software for the analysis and presenting of data generated during individual experiments. Computer software programs used for analysis and presentations include Microsoft Word, Excel, Power point, Adobe Photoshop, and Adobe Illustrator. Other programs that are specific to the analysis and designing of DNA reagents include FlowJo, DNA sequence analysis programs such as ApE, and gel analysis programs such as ImageJ. This position will report on a daily basis to Mr. Brian Ruis, the GESR (Genomic Engineering Shared Resource) Supervisor and/or to Drs. Eric Hendrickson or Branden Moriarity, Director and Co-Director of the GESR, respectively.
Job Duties: 50% TISSUE CULTURE Maintenance and propagation of human somatic cell lines. This project requires the extensive use of human cells in tissue culture and a significant portion of the applicant’s time will be used in the propagation, construction and maintenance of genetically engineered cell lines.
40% MOLECULAR BIOLOGY Perform experiments related to the construction of Cas9/CRISPR gene targeting reagents in addition, the applicant will need to be able to characterize at the DNA level, using PCR and DNA sequencing technology the genotypes of genetically modified cell lines.
10% DATA ANALYSIS Use computer software programs in the construction of gene targeting vectors and in the analysis of DNA sequencing data
BA/BS degree in Biology, Molecular Biology, or a related field plus 1 year of related experience, or a combination of related education and work experience related to the job totaling 5 years.
Experience with mammalian tissue culture, experience using CRISPR/Cas9 and experience with DNA DSB repair mechanisms required.
MA degree or experience with molecular biology in a lab setting preferred. Some experience with mammalian tissue culture preferred
Internal Number: 314954
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