Research - Laboratory/Non-Laboratory, Staff/Administrative
University of Minnesota - Division of Hematology, Oncology and Transplantation
This position involves the investigation of human immune tolerance, anti-tumor activity, and strategies to enhance tissue repair using mouse models. You will assist the lab by performing human immune cell experiments in vitro as well in animal models. This will include the use of multi-parameter flow cytometry to identify or purify rare immune cells. Human immune cells will also be used in functional assays to test various aspects of effector activity. You will also participate in caring for the NSG (immune deficient) mouse colony, dosing mice with experimental drugs/human cells/tumor cells as needed, scoring GVHD and tissue rejection (this can be taught), and performing mouse surgeries when required (typically human skin graft procedures onto NSG mice – this too can be taught). You will collaborate with researchers in the Betts Lab. Dr. Betts will provide guidance regarding experimental design, recording of endpoint data, proper procedure for clinical GVHD and skin graft rejection scoring, and how to perform human skin graft surgeries on NSG mice. The applicant will be expected to learn and be able to perform oral gavage, intraperitoneal injections, and tail vein injections of mice.
Job Duties/Responsibilities 50% - Human cell Culture and Bench Based Experiments This includes human cell culture, such as preparing T cell columns, generating specialized T cells or dendritic cells, or setting up various culture-based immune assays. Lab-specific assays will be taught if no prior experience. This also includes the staining of human cells for flow cytometry and the use of a flow cytometer to acquire data.
40% - Mouse Studies This includes transplanting human immune cells in immunocompromised mice to induce xenogeneic GVHD, GVHD scoring according to standard criteria, drug delivery of pharmacologic agents. Harvesting of specific tissues at set time points. Processing of human, split-thickness skin grafts, surgical grafting of human tissue to recipient mice, skin rejection scoring according to standard criteria, harvesting of specific tissues at set time points; and recording of data.
10% - Mouse Colony Maintenance This includes managing the Betts Lab mouse colony of NSG mice, such as housing breeding mice and weaning mice as needed. You will also be expected to monitor the health of the colony and research protocol mice on a regular basis.
Required Qualifications (Must be documented on application materials): -BA/BS and at least 1 year of flow cytometry experience or the equivalent combination of education and related work experience to equal five years - Previous experience in processing human blood cells (i.e. Ficoll or column separation kits) - Basic experience working with mouse/rodent research - Familiarity with human cell culture techniques - Strong ability to record data using Excel-style spreadsheets - Demonstrated high-level organization, attention to detail, and problem-solving skills - Excellent communications skills, both oral and written - Strong ability to follow instructions and clear objectives of the team - Willingness to work as a team, yet capable of handling situations independently
Preferred Qualifications - Experience performing functional human cell assays - Experience with staining cells and using a flow cytometer - Experience performing mouse surgeries - Knowledge of aseptic technique during mouse surgeries - Ability to work weekends to dose mice, when needed, with flexibility for weekday scheduling to accommodate such needs
Internal Number: 334194
About University of Minnesota, Twin Cities
The University of Minnesota, founded in the belief that all people are enriched by understanding, is dedicated to the advancement of learning and the search for truth; to the sharing of this knowledge through education for a diverse community; and to the application of this knowledge to benefit the people of the state, the nation, and the world.