Research - Laboratory/Non-Laboratory, Staff/Administrative
Summary of Position: The Center for Mass Spectrometry and Proteomics (CMSP) is seeking applications for the post of Junior Scientist to assist in the area of sample preparation. The successful candidate will work closely with the Sample Preparation Lead, other staff members and users of the facility to assist in sample preparation for proteomics and other biological mass spectrometry-based studies in a core service facility environment. Under supervision, this person will perform fundamental research experiments following established procedures. In addition, this person will perform routine lab maintenance, sample handling and potentially organize experimental data. This person will be expected to gain an understanding of laboratory operation to assist investigators as needed. Work requires precision and attention to detail. One should have strong written communication and interpersonal skills. Must have the ability to read and interpret direction, both oral and written, organize and prioritize work and follow through on assignments. The candidate must be able to work successfully as part of a team.
Essential and Other Functions: 50% - Mass Spectrometry Sample Preparation in-gel and in-solution trypsin digestion, protein extraction from various sample types, SDS-PAGE, solid phase extraction, other various sample prep duties. 35% - Quantitative Proteomic Sample Prep iTRAQ labeling, HPLC, and offline fraction concatenation prior to mass spectrometry. 15% - Other General Lab Duties and other duties as assigned.
Required Qualifications: Minimum of a bachelor’s degree in the field of biochemistry, chemistry or related field, experience with basic protein analysis methods, such as quantification assays, SDS-PAGE separations, gel staining and imaging, dialysis, and pipetting skills, basic computer skills, demonstrated ability to work well with others in a collaborative, team-based approach.
Preferred Qualifications: Knowledge of proteomic and/or metabolomic sample preparation for mass spectrometry, such as in-solution and in-gel proteolytic digestion, and peptide/protein purification methods, such as solid phase extraction (SPE) (e.g. C18 SPE, MCX SPE, zip-tipping, etc.), and precipitation methods. Working knowledge in the area of HPLC and biological mass spectrometry. Experience with more advanced proteomic sample preparation methods (e.g. labeling with TMT or iTRAQ reagent, immunoprecipitations, PTM enrichment, etc). Experience in training others in sample preparation methods.Experience in documenting protocols for dissemination to facility users.
Internal Number: 332057
About University of Minnesota, Twin Cities
The University of Minnesota, founded in the belief that all people are enriched by understanding, is dedicated to the advancement of learning and the search for truth; to the sharing of this knowledge through education for a diverse community; and to the application of this knowledge to benefit the people of the state, the nation, and the world.