Research - Laboratory/Non-Laboratory, Staff/Administrative
50% Responsible for the operational management of iPS core facility. This includes:
• Optimizing and modifying new technologies, space and resource utilization, hiring, supervising and managing Core facility staff, as well as providing core reagents and cells initially to those directly associated with the Core laboratory. • Technical and scienti c oversight of cell culture, DNA isolation engineering and analysis, media preparation, sorting, iPS generation and differentiation, genome engineering and molecular analyses. • Preparation of protocols and reports, including assimilating knowledge from literature and collaborators to apply to experimental design; • Development of assays and techniques and data analysis using variety of tools (e.g., statistics, bioinformatics). • Engages and meets with laboratories collaborating on human IPSC technology. Reports on progress, data, and obstacles in written and oral form.
25%: Writes manuscripts, sections of experimental design for grant proposals/grant reports and provides protocols applicable for human IPSC technology.
25% Supervise grad students, technicians and undergraduate students in or directly associated with the IPSC Core Facility and work in collaboration with postdocs and junior faculty. This position will develop and manage a state-of-the-art cellular reprogramming and differentiation Core Laboratory Service. The laboratory’s focus is in use of human induced pluripotent stem cells (IPSC) and the Manager will direct and oversee this effort, while also performing hands-on laboratory work as needed. A future goal of the Core facility is to advise and collaborate with other laboratories on optimizing the use of IPSC technology in an effort to develop new clinical therapies in a broad range of areas. Gene and cellular engineering approaches will be foundational techniques in the Lab, along with using animal models and precision gene targeting to modulate the transcriptional pro le of therapeutic cells genes. The work relies on CRISPR/Cas9 base and other gene targeting performed in a manner that promotes rapid translational application.
1. Advanced degree with at least 6 years of relevant research experience or a terminal degree plus at least 3 years of relevant research experience. 2. Extensive experience and skill in molecular biology, cellular engineering techniques and especially demonstrable success with generating, differentiating and/or genetically engineering human IPSCs. 3. Extensive experience and skill in cell isolation, culture, and ow and molecular analytic methodologies (i.e. intracellular FACS staining, PCR, and qRT PCR) 4. Responsible for development of and compliance with laboratory protocols, animal use regulatory standards, biological tissue handling & processing, and routine lab-related tasks. 5. Previous experience following written protocols and maintaining accurate and complete documentation of all procedures and experiments including demonstrated ability to readily perform all necessary laboratory projections, analysis and calculations. 6. Substantial track record of relevant research publications in peer review journals 7. Experience reporting effectively on the progress and direction of individual projects using written and verbal English, to include proper grammar usage and accurate spelling. 8. Demonstrated team player with the ability to adapt and lead a course of biological research. Strong communication skills are essential as this laboratory in the future will interact with a broad range of other laboratories at the UMN to provide advice and guidance in IPSC technology for developing new therapies.
1. Prior supervisory responsibilities 2. Prior experience with and expertise in induced pluripotent stem cell technology 3. Prior experience in training graduate students and postdocs
Internal Number: 331639
About University of Minnesota, Twin Cities
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