Research - Laboratory/Non-Laboratory, Staff/Administrative
This position is in the laboratory of Timothy Schacker under the guidance of Zachary Davis in the Department of Medicine. The employee will work on testing novel immunotherapeutic drugs for enhancing Natural Killer (NK) cell detection and killing of HIV infected cells using a variety of methods. This role entails the culture of HIV-infected primary cells as well as cell lines. Infected cells will be used to evaluate the ability of immunotheraputic drugs to augment the ability of natural killer cells to eliminate infected cells. Natural killer cells will be isolated from the blood of healthy donors and must be comfortable working with human blood roducts. Data will be collected primarily though the use of flow cytometry to identify changes in NK cell funciton. Potential applicants will be working with HIV infected cells in a biosafety level 3 facility with proper personal protective equipment (PPE). All responsibilities will be carried out with instruction and guidance from a supervisor.
Job Duties/Responsibilities 50% - Functional Assays/Flow Cytometry Functional assays to test NK cell functionality against HIV infected cells using a variety of treatments. All readouts will be carried out by flow cytometry and/or spectrophotometry.
20% - Blood Processing and Immune Cell Isolation Isolation and in vitro infection of primary human lymphocytes from whole blood
20% - Data Analysis Data analysis and report generation using a variety of software (FlowJo, PRISM, etc.)
10% - Tissue Cultures Long term tissue culture with mammalian cells (HIV infected and uninfected)
All required qualifications must be documented on application materials.
Required Qualifications -BA/BS in biology, genetics or related field with relevant coursework in immunology and cellular biology or a combination of related education and work experience to equal four years -Proficiency in cell culture and flow cytometry
-Adherence to all PPE requirements and proper handling of infectious agents
Preferred Qualifications -Previous experience in a research setting -Knowledge and practice of proper aseptic technique -Attention to detail and ability to troubleshoot problems -Good communication and technical writing skills
Internal Number: 329147
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