Research Support - Laboratory/Non-Laboratory, Staff/Administrative
We are looking for a highly motivated candidate specializing in Cryo-Electron Microscopy (Cryo-EM) to join the Structural Biology Core. The goal of this position will be to elucidate how intracellular membrane trafficking and remodeling processes, such as apoptosis and autophagy, are controlled and how dysregulation of these pathways may lead to cancer. The main focus is structure determination of multi protein complexes using Cryo-EM and single particle analysis. The new state-of-the-art Cryo-EM facility at The Hormel Institute is equipped with a FEI Titan Krios with phase plates and a direct electron detector. There is also a FEI Tecnai BT and an IBM cluster for image processing.
The successful candidate will assist facility staff with maintenance and operation of the advanced instrumentation house in the Cryo-EM core and will work with and train users in specimen preparation. These duties will involve a variety of complex laboratory tasks with standardized procedures. The candidate will also be responsible for record keeping associated with users, instrument logs, and routine operation of the facility.
This work will involve many of the stages of a structural biology project, from protein characterization and complex purification, to EM grid preparation. The candidate will also be expected to learn new procedures as they are developed and implemented by the core.
Job Duties and Responsibilities:
Facilitate and troubleshoot protein sample preparation – 60%
Assist users in assessing project feasibility.
Assist users in experimental design and data analysis.
Assist with instrument/microscope training and support – 15%
Preparation and screening of Cryo-EM samples – 10%
BA/BA/BS plus at least 4 years of experience or advanced degree plus 2 years of experience or a combo of related edu and work experience to equal 8 years
Doctorate Degree OR Master’s Degree and 4 years of scientific experience OR Bachelor’s Degree and 6 years of scientific experience.
Robust understanding of protein biochemistry.
Extensive hands-on experience in protein expression and purification using bacterial, insect or mammalian expression systems.
Knowledge of the principles of transmission electron microscopy and 3D structure determination.
Understanding of Cryo-EM workflow.
2-3 years of experience in Cryo-EM including handling samples, using microscopes and evaluating images and high-resolution single-particle analysis.
Demonstrated ability to learn new and innovative procedures quickly.
Experience with protein characterization methods, such as DSF, analytical ultra centrifugation, and size-exclusion chromatography.
Experience using and maintaining TEM microscopes, FEI series including Titan Krios, Talos Arctica.
Experience with Cryo-electron tomography.
Internal Number: 329085
About University of Minnesota, Twin Cities
The University of Minnesota, founded in the belief that all people are enriched by understanding, is dedicated to the advancement of learning and the search for truth; to the sharing of this knowledge through education for a diverse community; and to the application of this knowledge to benefit the people of the state, the nation, and the world.